Influence of Fungicide Application and Vine Age on Trichoderma Diversity as Source of Biological Control Agents

Fungi from the genus Trichoderma have a worldwide distribution and are commonly found in agricultural lands. Further, it has been described as non-virulent symbiont microorganism that can contribute to minimize pernicious effects of pathogens. In present work we isolated spp. bark grapevine different orchards order determine influence fungicide application vine age on diversity plant. An opposite correlation between number fungicides sprayed per campaign isolates was found. Moreover, older plants higher is isolates. The strains were tested against Phaeoacremonium minimum, trunk pathogen, evaluate their biocontrol capacity. Three shown significant capacity control P. minimum selected candidates be used biological agents. addition, rapid easy method for isolating developed, which allowed reduction amount pesticide use, together with preservation old vineyards, lead healthier agroecosystems containing levels beneficial microorganisms.